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R&D Systems anti mace 2
(A) Strategy: Vero-E6 cells were transduced with a lentiviral vector expressing <t>mACE-2</t> and a puromycin resistance gene. Cells were selected for mACE-2 expression by puromycin selection. (B) Expression in the selected polyclonal population was confirmed by Western blot.
Anti Mace 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Strategy: Vero-E6 cells were transduced with a lentiviral vector expressing mACE-2 and a puromycin resistance gene. Cells were selected for mACE-2 expression by puromycin selection. (B) Expression in the selected polyclonal population was confirmed by Western blot.

Journal: medRxiv

Article Title: The N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice and is neutralized by convalescent and post-vaccination human sera

doi: 10.1101/2021.01.19.21249592

Figure Lengend Snippet: (A) Strategy: Vero-E6 cells were transduced with a lentiviral vector expressing mACE-2 and a puromycin resistance gene. Cells were selected for mACE-2 expression by puromycin selection. (B) Expression in the selected polyclonal population was confirmed by Western blot.

Article Snippet: Anti-Tubulin and anti-mACE-2 (R&D Systems, Cat# MAB3437) primary antibodies were used at dilution of 1:1000 while secondary HRP-conjugated antibodies were used at dilutions of 1:10000 in 3% BSA-containing TBST.

Techniques: Transduction, Plasmid Preparation, Expressing, Selection, Western Blot